Correlative Light-Electron Microscopy (CLEM) L1-161
Unclaimed Principle — open for contribution
This Principle is declared in the catalog but has no reference solver, no pinned dataset, and is not registered on-chain. There is no reward pool. Submitting a cert against this Principle today will record the cert for reproducibility but pay zero PWM.
To claim it as a Bounty #7 contribution: open a PR adding (1) a reference solver, (2) ≥1 dataset pinned to IPFS, (3) updates to the L3 manifest with dataset CIDs. After verifier-agent triple-review, the founders' 3-of-5 multisig signs PWMRegistry.register() and the Principle becomes mineable.
Forward model E
Correlative Light-Electron Microscopy (CLEM): correlative light electron produces the measurement through a 4-node primitive DAG L.fluorescence_imaging -> L.em_imaging -> L.multi_scale_registration -> int.spatial, with spatially-projected accumulation and photon-shot-noise-limited (Poisson counting). Recovery is posed as a non-convex inverse problem that inverts the forward operator to estimate the scene-side 2D fluorescence plus em. Difficulty tier delta=5 with effective condition number kappa_eff~18; calibration-level mismatch (scale_mismatch, sample_preparation_artifact, registration_error) sets the accuracy floor at the Omega boundary. See the forward_model field for the closed-form imaging equation.
L-DAG
Well-posedness W
- Existence:
- true
- Uniqueness:
- true
- Stability:
- conditional
- κ:
- 360
Existence of the recovered 2D fluorescence plus em is guaranteed within the declared Omega bounds. Uniqueness is local rather than global (non-convex landscape); convergence depends on initialisation and priors. Stability is moderately conditioned (kappa_eff ~= 18); scale_mismatch dominates the stability cliff; sample_preparation_artifact and the remaining mismatch parameters contribute higher-order bias terms. Photon-shot-noise-limited (poisson counting) sets the irreducible data-fidelity floor, while TV / wavelet-sparsity / deep priors stabilise recovery at the ill-conditioned end of Omega.
Solvability C
- Solver class:
- linear-operator + convex optimisation [Fiducial-Register, Feature-Match] | linear-operator + deep neural prior [CLEM-Net]
- Convergence rate q:
- 2
- Complexity:
- O(H * W * log(...)) per iteration; learned variants: O(H W Z * F_theta_cost) per forward pass